Evaluation of microsatellite markers to discriminate induced mutation lines, hybrid lines and cultigens in chickpea (Cicer arietinum L)

Abstract

A total of 47 chickpea (Cicer arietinum L) accessions including 21 induced mutation lines, 17 hybrid lines, 5 local cultigens, and 4 non-nodulating lines were examined for their growth characteristics in the field. Genotypes of these accessions were also examined using 10 SSR (microsatellite) markers. Eight of the 10 markers were polymorphic and the number of alleles ranged from 2 to 16, with the average of 7.4 per locus. PIC values ranged from 0.227 to 0.876, with an average of 0.636. The accessions comprised 29 Desi and 18 Kabuli phenotypes. The average PIC was 0.582 in Desi and 0.577 in Kabuli phenotypes. Significant genetic differentiation was revealed between Desi …